Development and Application of Loop-mediated Isothermal Amplification Assay for Salmonella Detection in Fecal Samples of Experimental Monkeys

A loop-mediated isothermal amplification(LAMP)assay was developed for detection of Salmonllain fecal samples of experimental monkeys.According to the specific sequences(fimY)of Salmonllain GenBank,one set of primers was selected and the reaction condition was optimized.The results showed that the detection limit of LAMP method was 1.35×101 and 1.35×103 CFU/mL in Salmonellapure culture and clinical samples,respectively,which was the same as routine PCR.The amplification could complete in one hour,and the result could be distinguished by naked eyes.There was non-specific amplification of other pathogens.These results suggested that this LAMP assay was a simple and specific method for rapid detection of Salmonllain fecal samples.