RESEARCHES ON EXTRACTION METHOD FOR PIGMENT OF GLOBEFLOWER AND ITS STABILITY

When extracting the pigment of Globeflower by water, temperature, pH, and extraction frequency were respectively tested, based on it, the orthogonal test was conducted; the temperature and frequency of acetone extraction were also studied; furthermore the extracting ways were compared, the stability of pigment was explored. Under this experiment, the better factors for water extraction were 95 ℃~100 ℃, pH 7.0, one time for 30 min, for acetone extraction, at 54 ℃, one time lasting 30 min. The way of water- acetone extraction was superior to acetone-water extraction and single solvent. The pigment was steady under normal temperature, pH 3.0~7.0, saccharine medium. NaCl limited, low concentrations of Ca2+ and Mg2+ couldn't influence the pigment, Fe3+, strong sunlight and H2O2 could destroy pigment.