OBJECTIVE To understand the main reasons for cases in one hospital with the gastroenteritis symptoms so as to provide scientific references for prevention and control of enteric infectious diseases. METHODS The stools of ten patients were collected and detected by real-time PCR and sequencing for the viral detection, including rotavirus, norovirus, Sapporo virus, astrovirus, adenovirus. RESULTS Norovirus was detected in 7 out of 10 stools, with same subtype of GII.4. The results confirmed the etiology wasviral infection. CONCLUSION There is the threat of norovirus in Nanjing. Norovirus, subtype GII.4, is the pathogen, resulting in the gastroenteritis in the hospital. It is the same subtype of norovirus as in other countries; anti-virus advocacy should be strengthened on health education and especially for prevention of infection.