Loop-mediated isothermal amplification assay for rapid detection of Salmonella spp.

Salmonella spp.is an important kind of food-borne pathogenic bacteria which can cause human and animal disease.Salmonella invA gene is known as a major virulence gene.According to published Salmonella invA gene sequence in GenBank,we designed one sets of specific loop-mediated isothermal amplification primers and developed a novel and highly specific loop-mediated isothermal amplification assay for the sensitive and rapid detection of Salmonella spp.The assay correctly identified Salmonella spp.,but did not detect 7 non-Salmonella spp.strains.Sensitivity of the LAMP asssay for direct detection of Salmonella spp.in pure cul-tures was 1.04×102 cfu.mL-1.The results of LAMP detection of 100 eggs were consistent with the traditional method.The LAMP assay is a sensitive,rapid and simple tool for the detection of Salmonella spp.and will facili-tate the surveillance for control of contamination of Salmonella spp.in egg.