盐酸阿霉素
赫拉
透明质酸
细胞毒性
阿霉素
CD44细胞
介孔二氧化硅
药物输送
流式细胞术
化学
内吞作用
体外
生物物理学
材料科学
生物化学
分子生物学
纳米技术
细胞
介孔材料
生物
化疗
催化作用
遗传学
作者
Jing Zhang,Yan Sun,Baocheng Tian,Keke Li,Lele Wang,Yan Liang,Jingtian Han
标识
DOI:10.1016/j.colsurfb.2016.04.015
摘要
In this paper, a CD44-targeted and redox-responsive drug delivery system based on mesoporous silica nanoparticles (MSNs) was synthesized by conjugating tumor-shedable hyaluronic acid (HA) on the surface of MSNs via disulfide bonds. Doxorubicin hydrochloride (DOX·HCl) was physically encapsulated into HA modified MSNs (MSNs/SS/[email protected]) as a model drug. MSNs/SS/[email protected] (40 nm) had a high drug loading (14.1%) and redox-responsive drug release property. The cellular uptake behaviors of MSNs/SS/[email protected] by HeLa and LO2 cells were evaluated by confocal laser scanning microscopy (CLSM) and flow cytometry (FCM). MSNs/SS/[email protected] exhibited higher cellular uptake efficacy via CD44-mediated endocytosis by HeLa cells (CD44 over-expressed cells) than by LO2 cells (CD44 deficient cells). The in vitro cytotoxicity assay demonstrated that MSNs/SS/[email protected] exhibited higher cytotoxicity to HeLa cells than to LO2 cells. These results indicated that MSNs/SS/[email protected] might be promising as a multifunctional drug delivery system to improve the anti-tumor efficacy of chemotherapeutic drugs.
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