In this paper,bran-kojis with single-strains and mixed strains were used to produce Baoning vinegar with solid-state fermentation.The results showed that strains and mixed mode had significant effect on the activity of enzyme,utilization of starch and vinegar yield.The utilization of starch and vinegar yield produced by mixed bran-kojis containing strain AS3.4309 and AU052 were increased by 38.72% and 21.22%,respectively,compared with those of traditional solid-state vinegar fermentation.