化学
适体
铁氰化物
介电谱
检出限
生物传感器
圆二色性
乙醇胺
自来水
光谱学
电化学
电极
色谱法
分析化学(期刊)
无机化学
结晶学
物理化学
生物化学
物理
工程类
环境工程
生物
量子力学
遗传学
作者
Gang Liang,Yan Man,Xinxin Jin,Ligang Pan,Xinhui Liu
标识
DOI:10.1016/j.aca.2016.06.056
摘要
A label-free sensing assay for ethanolamine (EA) detection based on G-quadruplex-EA binding interaction is presented by using G-rich aptamer DNA (Ap-DNA) and electrochemical impedance spectroscopy (EIS). The presence of K+ induces the Ap-DNA to form a K+-stabilized G-quadruplex structure which provides binding sites for EA. The sensing mechanism was further confirmed by circular dichroism (CD) spectroscopy and EIS measurement. As a result, the charge transfer resistance (RCT) is strongly increased as demonstrated by using the ferro/ferricyanide ([Fe(CN)6]3−/4−) as a redox probe. Under the optimized conditions, a linear relationship between ΔRCT and EA concentration was obtained over the range of 0.16 nM and 16 nM EA, with a detection limit of 0.08 nM. Interference by other selected chemicals with similar structure was negligible. Analytical results of EA spiked into tap water and serum by the sensor suggested the assay could be successfully applied to real sample analysis. With the advantages of high sensitivity, selectivity and simple sensor construction, this method is potentially suitable for the on-site monitoring of EA contamination.
科研通智能强力驱动
Strongly Powered by AbleSci AI