Bacterial cell differentiation during plant root colonization: the putative role of fructans

Plant-growth-promoting microorganisms are extensively studied and employed as alternatives to toxic agrochemicals to enhance plant health. However, one of the main concerns regarding their use is their limited capacity to colonize plant tissues after initial application. Understanding the molecular mechanisms involved during plant colonization could help to develop strategies to improve the efficacy of beneficial microbes in the field. Polysaccharides, including fructans, may be of particular interest since they have been shown to promote cellular and morphological changes in bacteria from the genus Bacillus that are typically associated with improved root colonization, such as increased motility and biofilm reinforcement. The potential role of fructans as signalling molecules affecting plant-microbe interactions is discussed in the context of plant root colonization with a focus on the model organism Bacillus subtilis, a well-characterized rhizobacterium. First, the molecular processes underlying B. subtilis cell differentiation are explained and connected to plant root colonization. Secondly, we explore how fructans, in particular inulin and levan, may interfere during these processes. These views call for further research into the putative role of inulin and levan-type fructans as microbial signalling molecules, with the aim of developing beneficial microbial networks in the rhizosphere.