Flow fermentation: microsystems for whole-cell bioproduction processes

Industrial biotechnology utilizes whole cells for the production of value-added goods in large-scale bioreactors. The miniaturization of bioreactors has greatly contributed to the understanding and optimization of bioprocesses. However, microsystems for the production of value-added goods have thus far only been established in chemistry and biocatalysis/biotransformation but are rarely applied for whole-cell bioprocesses. Here, we discuss the fundamental and translational aspects of how microsystems could be used as production units for future whole-cell bioproduction processes. The characteristics and resulting advantages of microsystems are introduced and current production approaches are highlighted. Finally, we provide perspectives on establishing future whole-cell bioproduction processes at the microscale, here introduced as flow fermentation. Flow fermentation potentially enables entirely new bioprocesses and application fields.