共价键
检出限
纳米颗粒
生物传感器
钌
滚动圆复制
电化学发光
化学
核酸外切酶 III
DNA
核酸外切酶
材料科学
适体
组合化学
纳米技术
DNA聚合酶
色谱法
有机化学
催化作用
分子生物学
生物化学
基因
大肠杆菌
生物
作者
Jiang Pan,Hai‐Liang Zhu,Jun Chen,Xiaoqi Ma,Ai-Jun Wang,Pei-Xin Yuan,Jiu‐Ju Feng
出处
期刊:Talanta
[Elsevier]
日期:2024-07-01
卷期号:274: 125934-125934
标识
DOI:10.1016/j.talanta.2024.125934
摘要
Nowadays, novel and efficient signal amplification strategy in electrochemiluminescence (ECL) platform is urgently needed to enhance the sensitivity of biosensor. In this work, the dual ECL signal enhancement strategy was constructed by the interactions of Pd nanoparticles attached covalent organic frameworks (Pd NPs@COFs) with tris (bipyridine) ruthenium (RuP) and Exonuclease III (Exo.III) cycle reaction. Within this strategy, the COFs composite was generated from the covalent reaction between 2-nitro-1,4-phenylenediamine (NPD) and trialdehyde phloroglucinol (Tp), and then animated by glutamate (Glu) to attach the Pd NPs. Next, the "signal on" ECL biosensor was constructed by the coordination assembly of thiolation capture DNA (cDNA) onto the Pd NPs@COFs modified electrode. After the aptamer recognition of progesterone (P4) with hairpin DNA 1 (HP1), the Exo. III cycle reaction was initiated with HP2 to generate free DNA, which hybridized with cDNA to form double-stranded DNA (dsDNA). For that, the RuP was embedded into the groove of dsDNA and achieved the ultrasensitive detection of P4 with a lower limit of detection (LOD) down to 0.45 pM, as well as the excellent selectivity and stability. This work expands the COFs-based materials application in ECL signal amplification and valuable DNA cyclic reaction in biochemical testing field.
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