[Regulative effect of active components of Cistanche deserticola on intestinal dysbacteriosis induced by antibiotics in mice].

Objective: To study the effects of Cistanche deserticola and its active components Cistanche deserticola polysaccharide and Echinacoside on intestinal flora of antibiotic-associated diarrhea (AAD) mice. Methods: Forty-eight Balb/c mice were randomly divided into control (Con) group, AAD Group, inulin (Inu) group, Cistanche deserticola (RCR) group, Cistanche deserticola polysaccharide (RCRDT) group and Echinacoside (Ech) group with 8 mice in each group. The diarrhea model of mice was induced by intragastric administration of lincomycin hydrochloride(3 g/kg) for 7 days, and then treated by intragastric administration of INU(5 g/kg), RCR(5 g/kg), RCRDT(200 mg/kg) and ECH (60 mg/kg),0.2 ml once a day for 7 days, Con group and AAD group were given the same volume of normal saline. By observing general signs of mice, colon HE staining, 16S rDNA high-throughput sequencing analysis, the effects of Cistanche deserticola, Cistanche deserticola polysaccharide and Echinacea glycoside on the imbalance of intestinal flora induced by antibiotics in mice were evaluated. Results: Compared with Con group, AAD group mice lost weight, presented obvious diarrhea symptoms, inflammatory changes in colon tissue and decreased intestinal flora diversity (P＜0.05) indicating the success of the model. Compared with AAD group, the weight and diarrhea of INU, RCR, RCRDT and ECH groups were significantly improved, and the colon pathology of ECH group was restored to normal level. Compared with AAD group, RCR group, RCRDT group and ECH group had significantly decreased intestinal Firmicutes, increased Blautia and Lachnoclostridium, and decreased Clostridium_sensu_stricto_1 (P＜0.05) . In ECH group, the abundance and diversity of intestinal microflora were returned to normal level, and the structure of intestinal microflora was well adjusted, the contents of Bacteroides,Flavonifractor,Agathobacter,Lachnoclostridium and Prevotella-9 were increased (P＜0.01). Conclusion: Both Cistanche deserticola and its active components cistanche deserticola polysaccharide and echinacoside can regulate the intestinal flora imbalance caused by antibiotics and improve the symptoms of AAD, especially echinacoside.目的: 研究肉苁蓉及其有效成分肉苁蓉多糖和松果菊苷对抗生素相关性腹泻(AAD)小鼠肠道菌群的影响。方法: 48只Balb/c小鼠随机分为对照(CON)组、AAD组、菊粉(INU)组、肉苁蓉(RCR)组、肉苁蓉多糖(RCRDT)组和松果菊苷(ECH)组,每组8只,连续7 d灌胃盐酸林可霉(3 g/kg)造小鼠腹泻模型,随后灌胃INU(5 g/kg)、RCR(5 g/kg)、RCRDT(200 mg/kg)和ECH(60 mg/kg)各0.2 ml,1次/日,治疗7 d,CON组和AAD组小鼠灌胃同体积的生理盐水。通过观察小鼠一般体征、结肠HE染色、6S rDNA高通量测序分析,综合评价肉苁蓉及其有效成分肉苁蓉多糖和松果菊苷对抗生素所致小鼠肠道菌群失调的作用。结果: 与CON组相比,AAD组小鼠体重减轻,出现明显腹泻症状,结肠组织出现炎性改变,肠道菌群多样性降低(P＜0.05),说明造模成功。与AAD组相比,INU组、RCR组、RCRDT组和ECH组小鼠体重恢复,腹泻症状明显改善;ECH组小鼠结肠病理恢复至正常水平;与AAD组相比,RCR组、RCRDT组和ECH组小鼠肠道厚壁菌门含量明显减少,Blautia和Lachnoclostridium含量增加,Clostridium_sensu_stricto_1含量减少(P＜0.05),此外,ECH组小鼠肠道菌群丰度和多样性恢复至正常水平,肠道菌群结构得到良性调整,拟杆菌属、Flavonifractor、Agathobacter、Lachnoclostridium、Prevotella-9等含量增加(P＜ 0.01)。结论: 肉苁蓉及其有效成分肉苁蓉多糖和松果菊苷均可以调节抗生素所致肠道菌群失调,改善AAD症状,其中松果菊苷的作用更明显。.