化学
活力测定
过氧化氢酶
MTT法
酶
氧化还原酶
循环伏安法
咪唑酯
生物物理学
生物化学
核化学
电化学
细胞
无机化学
生物
物理化学
电极
作者
Tapan Dey,Netra Hiremath,Vishav Kant,Rakesh K. Sharma,Raviraj Vankayala,Saikat Dutta
出处
期刊:Biomaterials Science
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:11 (15): 5136-5145
被引量:1
摘要
In the present study, we sought to reveal how embedding oxidoreductase enzymes in a metal-organic framework influences restoring the biofunctionality when encapsulated within zeolitic imidazolate framework (ZIF-8 and ZIF-90), wherein these biocomposites were explored for their cellular metabolic activity using the (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) (MTT) assay on A549 lung cancer cells and NIH3T3 (mouse fibroblasts) cells. We chose two biocomposites, namely catalase-encapsulated ZIF-8 and ZIF-90, wherein the enzyme was encapsulated at varied loadings through a rapid self-triggered nucleation around the protein surfaces of the enzyme. Interestingly, this embedding pattern of catalase in both ZIF-8 and ZIF-90 depended on the surface chemistry of the enzyme. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy analysis revealed the stability of the encapsulated enzyme in the nanospace of the ZIF-8 and ZIF-90 frameworks. Investigation of the cellular metabolic activity by the MTT assay of Cat@ZIF-8 and Cat@ZIF-90 on the lung cancer cell A549 showed cell viability enhancement in the case of Cat@ZIF-8 at a higher percentage compared to that of Cat@ZIF-90. A similar metabolic activity assay was performed with the internalization of Cat@ZIF-90 for NIH3T3 (mouse fibroblasts) cells. The revealed difference between the MOF compounds was due to the nano-confinement effect in ZIF-8 compared to ZIF-90, which can accelerate the utilization in cellar metabolic activity.
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