Straightforward fabrication of electrochemical aptasensors with outstanding antifouling performance

Self-assembled monolayers (SAMs) are popular tools for many different applications - SAMs of commercially available chemicals that convincingly inhibit unspecific binding for electrochemical sensors, however, have yet to be developed. While adsorption of foulants prohibits the reliable analysis of biological samples, unspecific binding of the analyte similarly impedes the investigation of binding characteristics from buffer solutions. In this communication, diglycolamine is introduced for the modification of electrodes with outstanding antifouling performance. The presented sensor design, solely consisting of diglycolamine and an aptamer of choice, convinces with its ease of preparation, low cost, and, most importantly, an exceptional specificity. The latter was found to rely on a gentle but potent cleaning of the electrodes, as only our optimized cleaning procedure granted the diglycolamine layer its excellent fouling minimization performance, while literature standard protocols failed to do so. Each step of the sensor fabrication protocol was optimized by electrochemical impedance spectroscopy, while square-wave voltammetry, surface-enhanced Raman spectroscopy, and zeta potential measurement were performed for further characterization. The presented approach of surface modification with diglycolamine is a versatile method applicable not just to electrochemical measurements, but to a variety of other detection techniques, too, and has the potential to change the way we investigate binding characteristics and fabricate sensors for the analysis of complex biological samples.