Utilization of an aqueous two-phase emulsification to prepare bimodal porous cellulose monolith for efficient adsorption of bovine serum albumin

Cellulose monolith has garnered significant interest in the field of biochromatography, which lies in its interconnected porous structure, large surface area and biocompatibility. In this context, we propose a novel approach for preparing cellulose monoliths using an aqueous two-phase system devoid of any organic solvents and surfactants. In this strategy, emulsifying cellulose solution into PEG 20,000 solution gives bicontinuous aqueous phases and further porous cellulose monolith after regeneration of dissolved cellulose. And the macroporous channels are derived from the removal of the PEG 20,000 aqueous phase while the micropores are from the phase separation of the cellulose phase. Physical characterizations reveal the obtained cellulose monolith exhibits exceptional column permeability of 1.36 × 10-11 m2 and a substantial surface area of 39.34 m2/g. Furthermore, cellulose monolith is functionalized with diethyl ethylamine hydrochloride (DEAE-HCl) to evaluate its potential as an anion adsorbent. Experimental results reveal that the DEAE-modified cellulose monolith possesses of adsorptive capacity of 316.58 mg/g of bovine serum albumin, along with fast adsorption kinetic. This study introduces an innovative strategy for fabricating porous cellulose monoliths tailored for biochromatography applications.