脂肪酶
材料科学
介电谱
基质(水族馆)
生物传感器
脂质双层
电极
膜
纳米技术
化学
生物化学
酶
生物
生态学
物理化学
电化学
作者
Upeksha Mirissa Lankage,Stephen A. Holt,Samara Bridge,Bruce Cornell,Charles G. Cranfield
标识
DOI:10.1021/acsami.3c11767
摘要
Sensors that can quickly measure the lipase activity from biological samples are useful in enzyme production and medical diagnostics. However, current lipase sensors have limitations such as requiring fluorescent labels, pH control of buffer vehicles, or lengthy assay preparation. We introduce a sparsely tethered triglyceride substrate anchored off of a gold electrode for the impedance sensing of real-time lipase activity. The tethered substrate is self-assembled using a rapid solvent exchange technique and can form an anchored bilayer 1 nm off the gold electrode. This allows for an aqueous reservoir region, providing access to ions transported through membrane defects caused by triglyceride enzymatic hydrolysis. Electrical impedance spectroscopy techniques can readily detect the decrease in resistance caused by enzymatically induced defects. This rapid and reliable lipase detection method can have potential applications in disease studies, monitoring of lipase production, and as point-of-care diagnostic devices.
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