UMPPI: Unveiling Multilevel Protein–Peptide Interaction Prediction via Language Models

Protein-peptide interactions are essential to cellular processes and disease mechanisms. Identifying protein-peptide binding residues is critical for understanding peptide function and advancing drug discovery. However, experimental methods are costly and time-intensive, while existing computational approaches often predict interactions or binding residues separately, lack effective feature integration, or rely heavily on limited high-quality structural data. To address these challenges, we propose UMPPI (Unveiling Multilevel Protein-Peptide Interaction), a multiobjective framework based on the pretrained protein language model ESM2. UMPPI simultaneously predicts binary protein-peptide interactions and binding residues on both peptides and proteins through a multiobjective optimization strategy. By integrating ESM2 to encode sequences and extract latent structural information, UMPPI bridges the gap between sequence-based and structure-based methods. Extensive experiments demonstrated that UMPPI successfully captured binary interactions between peptides and proteins and identified the binding residues on peptides and proteins. UMPPI can serve as a useful tool for protein-peptide interaction prediction and identification of critical binding residues, thereby facilitating the peptide drug discovery process.