期限(时间)
生物
维甲酸
细胞生物学
减数分裂
营养物
维甲酸
遗传学
生态学
细胞培养
基因
物理
量子力学
作者
Xiaoyu Zhang,Ning Wang
出处
期刊:Methods in molecular biology
日期:2024-01-01
卷期号:: 113-121
标识
DOI:10.1007/978-1-0716-3698-5_9
摘要
Spermatogonial stem cells (SSCs) produce haploid sperm via mitosis and meiosis in vivo. Although the technique to culture mouse SSCs has been well established, induction of meiosis in vitro has remained a challenge. Retinoic acid (RA) is required for meiosis in vivo; however, RA alone is not sufficient to induce meiosis in vitro. Here, we describe a method in which nutrient restriction and RA synergistically induce meiotic initiation into meiotic prophase I in cultured mouse SSCs.
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