双歧杆菌
莲花
体内
益生元
拟杆菌
莲花效应
食品科学
长双歧杆菌
体外
化学
发酵
肠道菌群
生物
生物化学
乳酸菌
植物
细菌
原材料
遗传学
生物技术
有机化学
作者
Xiang Ma,Zheng Zhou,Qi Wang,Jiaxin Zuo,Jianing Ju,Baodong Zheng,Xu Lu
标识
DOI:10.1016/j.foodchem.2023.136057
摘要
Natural lotus seed oligosaccharides monomers (LOSs: LOS3-1, LOS3-2, and LOS4) were prepared by preparative chromatography and were hydroxyl-labeled with fluorescein isothiocyanate (FITC). The prebiotic properties of LOSs by the gut microbiota of male Balb/C mice in vivo and in vitro were studied. In vivo experiment results showed that LOS4 could significantly increase the average daily food consumption, weight, liver index and the abundance of Bacteroides and Bifidobacterium for mice (p < 0.05). In addition, LOS4 also had significant proliferation effect on Bifidobacterium adolescentis and longum in vitro (p < 0.05). Laser confocal microscopy observation showed interaction site between LOS4-FITC and Bifidobacterium adolescentis was located outside and inside of cell, which was completed within 1 h. The relationship between structures of LOSs and prebiotics of intestinal flora (especially Bifidobacterium), and expanded the knowledge on the effects of carbohydrate polymerization degree (DP) and glycosidic bond connection with fermentation selectivity of bacteria was studied.
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