Advances in Fungal Promoter Engineering for Enhancing Secondary Metabolite Biosynthesis

ABSTRACT In the past two decades, promoter engineering has been extensively employed as a powerful strategy for fine‐tuning the regulation of gene transcription to awaken or increase the expression of secondary metabolite (SM) biosynthetic gene clusters (BGCs) in fungal genomes. This comprehensive review provides an overview of recent advances in various fungal promoter engineering techniques, including promoter replacement, optimization, and combinatorial methods, and their application in the enhancement of SM production. Additionally, current challenges and future prospects for efficient promoter engineering for precise metabolic regulation in fungi are discussed.