Evaluation of IL-1RA, IL-1β IFN-γ and CXCL-10 as mediators of damage and hepatic repair in chronic hepatitis and fibrosis progresion

Background and aim: Intestinal permeability increases in alcoholics allowing the passage of lipopolysaccharide (LPS) to liver, promoting TLR-4 activation in Kupffer cells and the production of pro-inflammatory cytokines.At the present, the modulation of LPS in alcoholics at systemic level, is not fully understood.Aim.To evaluate TLR-4 in M1 monocytes and the production of IL-6 and CXCL-8 in alcoholic liver disease.Material and methods: Cross-sectional study, that include Alcoholic patients (according WHO) from the Liver clinic of the General Hospital of Mexico were included.They were classified by absence (OH) or presence (CiOH) of liver damage and patients with active alcoholic hepatitis (HOH).Control group (CT): AUDIT <8 and intake of <10gOH / day.Blood samples were taken on one occasion (10 ml) to obtain mononuclear cells by density gradient.To which cell marking was performed by flow cytometry (M1 and TLR-4) and in serum was performed the determination of cytokines (IL-6 and CXCL-8) by arrangement in multiple suspension.Mann Whitney U statistical analysis.All patients signed informed consent.Protocol