Kinetics of Sophora japonica embryo cells in a suspension culture system CHEN Hongxian, YU Xiaoxiao, WANG Chenyang, ZHANG Min, LIU Zhonghua

Based on the established cell suspension culture system of Sophora japonica embryos, its kinetics was investigated. The suspension cell system of S. japonica embryos influenced by inoculum size was studied with analysis during cell culture of kinetic parameters, such as cell growth; consumption of C, N, and P; as well as changes in cell fresh weight, dry weight, and flavonoid production at various stages in the culture procedure. Results revealed that. （1） the preferable inoculum size for suspension cell proliferation was 40.000 gL-1. （2） The cell suspension culture cycle lasted about 27 d with maximum biomass in dry weight reaching 9.733 gL-1 and flavonoid production attaining 53.566 mgL-1. Flavonoid production was semi-associated with cell growth of the S. japonica embryo being negatively related from 6-12 d and positively related from 12-33 d. （3） From 0-6 d sugar, N, and phosphate were absorbed quickly; after 12 d they were more than 80% absorbed. Also, NH4+ was utilized faster than NO3-. Thus, proliferation and flavonoid production of S. japonica embryo cells was closely related to the consumption of sugar, N, and phosphate. This research could provide references for the scale-up culture of S. japonica embryos cell by bioreactor.［Ch, 10 fig. 25 ref.］