溶酶体
化学
荧光
细胞凋亡
生物物理学
纳米技术
碳纤维
生物化学
酶
材料科学
量子力学
生物
复合数
物理
复合材料
作者
Haoyue Qin,Yuanqiang Sun,Xin Geng,Keran Zhao,Hong-Min Meng,Ran Yang,Lingbo Qu,Zhaohui Li
标识
DOI:10.1016/j.aca.2020.02.002
摘要
In this work, a novel bright yellow fluorescent carbon dots (CDs) was synthesized from N-methyl-1,2-phenylenediamine hydrochloride in ethanol solvent through the solvothermal method. The obtained carbon dots had no fluorescence in water, but could specifically light up in the cell which makes the staining process without washing. Interestingly, the imaging process can be performed by simply shaking the culture with the cells for only 1 min, indicating ultrafast and easy to operate fluorescence imaging. Moreover, the carbon dots with abundant amino functional groups had good lysosome targeting properties (the Pearson’s correlation coefficient is 0.92) and could achieve the ultrafast lysosome imaging in cell and zebrafish and monitoring cell apoptosis status. This is the first lysosome targeting carbon dots that allows the combination of a short incubation at the second-level with wash-free process providing great potential for continuous observation lysosome in vivo.
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