罗亚
细胞生物学
巨噬细胞
生物
吞噬作用
效应器
肌动蛋白细胞骨架
运动性
极性(国际关系)
移植
Rho相关蛋白激酶
电池极性
肌动蛋白
表型
小型GTPase
免疫系统
GTP酶
细胞骨架
免疫学
信号转导
体外
细胞
生物化学
医学
内科学
基因
作者
Yianzhu Liu,Neelam Tejpal,Junping You,Xian Chang Li,Rafik M. Ghobrial,Małgorzata Kloc
标识
DOI:10.1016/j.cellimm.2015.12.005
摘要
Macrophages play an important role in immune responses including allograft rejection and they are one of the potential targets of anti-rejection therapies in organ transplantation. Macrophage alloreactivity relies on their phenotype/polarity, motility, phagocytosis and matrix degradation, which in turn depend on proper functioning of actin cytoskeleton and its regulators, the small GTPase RhoA and its downstream effector the Rho-associated protein kinase (ROCK). Several laboratories showed that administration of ROCK inhibitor Y-27632 to the graft recipient inhibits chronic rejection or rodent cardiac allografts. Here we studied the effect of Y-27632 on mouse peritoneal macrophage structure, polarity and functions in in vitro assays. We show that Y-27632 inhibitor affects macrophage phenotype/polarity, phagocytosis, migration, and matrix degradation. These novel findings suggest that the impediment of macrophage structure and function via interference with the RhoA/ROCK pathway has a potential to be therapeutically effective in organ transplantation.
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