Electrical Communication between Glucose Oxidase and Electrodes Based on Poly(vinylimidazole) Complex of Bis(2,2′-bipyridine)-N,N′-dichloroosmium

Glucose oxidase (GOX) was electrically 'wired' to glassy carbon electrodes by redox hydrogels based on poly (N-vinyl imidazole) (PVI) complexed with Os(bpy)2Cl2 (PVI-Os). The hydrogels were formed by crosslinking PVI-Os and glucose oxidase with poly(ethylene glycol) diglycidyl ether (PEGDGE). Glucose was electrooxidized on the PVI-Os based 'wired' enzyme electrodes at 350 mV vs SCE. The dependence of the electrooxidation current on pH, ionic strength, film thickness, weight fraction of the enzyme in the redox hydrogel and oxygen partial pressure for electrodes rotating at 1000 rpm is described.