检出限
荧光
抗坏血酸
激进的
光化学
石墨烯
化学
量子点
过氧化氢
苯醌
线性范围
核化学
材料科学
色谱法
有机化学
纳米技术
物理
量子力学
食品科学
作者
Hua Liu,Weidan Na,Ziping Liu,Xueqian Chen,Xingguang Su
标识
DOI:10.1016/j.bios.2017.02.005
摘要
In this paper, a facile and rapid fluorescence turn-on assay for fluorescent detection of ascorbic acid (AA) was developed by using the orange emission graphene quantum dots (GQDs). In the presence of horse radish peroxidase (HRP) and hydrogen peroxide (H2O2), catechol can be oxidized by hydroxyl radicals and converted to o-benzoquinone, which can significantly quench the fluorescence of GQDs. However, when AA present in the system, it can consume part of H2O2 and hydroxyl radicals to inhibit the generation of o-benzoquinone, resulting in fluorescence recovery. Under the optimized experimental conditions, the fluorescence intensity was linearly correlated with the concentration of H2O2 in the range of 3.33–500 µM with a detection limit of 1.2 µM. The linear detection for AA was in the range from 1.11 to 300 µM with a detection limit of 0.32 µM. The proposed method was applied to the determination of AA in human serum samples with satisfactory results.
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