The Effect of MicroRNA-92a (miR-92a) on Non-Small Cell Lung Cancer (NSCLC) Cell Biological Behaviors and Janus Protein-Tyrosine Kinase 1 (JAK1)/Signal Transducer and Activator of Transcription (STAT3) Signaling

Our study investigates miR-92a’s effect on the biological behaviors of non-small cell lung cancer (NSCLC) cells and Janus protein-tyrosine kinase (JAK)/signal transducer and activator of transcription (STAT) signal transduction. A549 cells were divided into KB group (no transfection), NC group (negative control transfection), and SY group (transfection with miR-92a inhibitor) followed by analysis of the expression of miR-92a, JAK1, and STAT3 by qRT-PCR, cell proliferation, invasion and apoptosis as well as JAK1/STAT3 protein expression. The miR-92a, JAK1, and STAT3 expression in SY group were significantly lower than NC group and KB group ( P <0.05), indicating the successful transfection. The A549 cell proliferation in SY group was significantly decreased compared with NC and KB group ( P <0.05). Cell apoptosis rate in SY group was 25.23±2.31%, which was significantly higher than that in NC (8.15±0.82%) and KB group (8.08±0.79%). The number of cell invasion in SY group was significantly reduced (88.6±7.32) compared with NC group (189.71±15.37) and KB group (181.32±14.62) ( F = 937.8, P <0.001). SY group showed significantly lower JAK1/STAT3 protein expression than NC and KB group. In conclusion, silencing miR-92a can inhibit proliferation, migration, and invasion of A549 cells, which may be related to JAK1/STAT3 signaling pathway.