Influence of glucose, sucrose, and dextran coatings on the stability and toxicity of silver nanoparticles

Aqueous colloids, consisting of 15-30 nm-sized silver nanoparticles (Ag NPs), were prepared using the reducing and stabilizing abilities of glucose, sucrose, and dextran. The long-term stability of coated Ag NPs increases from glucose over sucrose to dextran, i.e., with the increase of the molecular weight of carbohydrate molecules. The density functional theory (DFT) calculations of the partial atomic (Mulliken) charges and adsorption energies are applied to explain the enhanced stability of coated Ag NPs. All coated Ag NPs have a significantly broader concentration range of nontoxic behavior toward pre-osteoblast cells than bare Ag NPs prepared using sodium borohydride. The carbohydrate-coated Ag NPs display the same level of toxic ability against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria as bare Ag NPs. The differences in toxicity mechanism of the coated and bare Ag NPs are a consequence of the absence and presence of co-occurring Ag