Selective detection of Asulam with in-situ dopamine electropolymerization based electrochemical MIP sensor

In this work, a new method is presented based on in-situ electropolymerization of dopamine (DA) monomer and asulam (ASL) as a target molecule to fabricate a molecularly imprinted polymer (MIP) on a glassy carbon electrode (GCE). In this regard, the most basic feature of the prepared sensor is that after template extraction the target molecule, cavities with the same size and stereochemical properties of it, remain on the imprinted polymer matrix. Accordingly, the developed sensing platform showed high selectivity and great sensitivity in ASL detection. The proposed method showed good linear range area from 0.5 to 20 pM with limit of detection of 0.17 pM. The influences of electropolymerization conditions e.g. incubation time, monomer to template concentration, cycle numbers and template extraction time were studied. Finally, it was applied for ASL detection in real samples, and the results were consistent with high-performance liquid chromatography obtained results.