L-rhamnose as a trace sugar is widely distributed in plants,it's one of the common sweeteners in food processing,it also can be used in the production of flavor and fragrance. The study found that L-rhamnose induced yeast mannan can improve α-mannosidase activity in animal body,and show a good medicinal value. On the one hand the research situation about preparation method and application of L-rhamnose was introduced,on the other hand,according to the chemical properties and determination principle of L-rhamnose,combined with the latest achievements,the detection methods of L-rhamnose were reviewed,qualitative,quantitative detection method of L-rhamnose were introduced from aspects of TLC,HPLC,DNS and other aspects,the advantages and disadvantages of various detecting technologies were compared.