溶菌酶
动态光散射
化学
圆二色性
纤维
静态光散射
变性(裂变材料)
淀粉样蛋白(真菌学)
水溶液
蛋白质聚集
生物物理学
结晶学
材料科学
纳米颗粒
生物化学
核化学
纳米技术
有机化学
无机化学
生物
作者
Isala Dueramae,Shingo Fukuzawa,Naoki Shinyashiki,Shin Yagihara,R. Kita
出处
期刊:Journal of Biorheology
[Japanese Society of Biorheology]
日期:2017-01-01
卷期号:31 (1): 21-28
被引量:2
摘要
We investigated the mechanisms of amyloid-like aggregation and gel formation in hen egg-white lysozyme (HEWL) in a mixed solvent comprising 90% v/v ethanol in water using dynamic light scattering (DLS) and circular dichroism CD. The mechanism of HEWL in ethanol aqueous solution is interpreted into three stages as: (I) denaturation of HEWL; (II) elongation of amyloid fibrils composed of β-sheet-rich HEWL by lateral aggregation; and (III) gel formation due to the creation of junctions in amyloid fibrils. The transformation of sol to gel can be confirmed by: (1) the oscillation behavior and the rapid increase in the intensity of scattered light; (2) the power-law behavior of the correlation function of scattered light g(2)(t); (3) the appearance of a long-time tail in the distribution function of the decay time G(τ); and (4) the beginning of the reduction in initial amplitude in g(2)(t). The gelation rate was strongly dependent on the protein concentration. The estimated rod length of the amyloid fibrils increased significantly over time. Scanning electron microscopy (SEM) performed on the formation of fibrils in the HEWL gels revealed that the structure was highly heterogeneous, with areas characterized by dense fiber networks interspersed with loose network areas.
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