Antibacterial activity againstRalstonia solanacearumof the lipopeptides secreted from theBacillus amyloliquefaciensstrainFJAT‐2349

AIMS: The aims of this study were to identify the structure of antibacterial lipopeptide (LP) produced by Bacillus amyloliquefaciens strain FJAT-2349, to analyse the effects of the culture medium and temperature on LP production and to assess the biocontrol efficiency of the LPs against tomato bacterial wilt. METHODS AND RESULTS: under different culture temperatures respectively. The results indicated that the contents of the LP mixture, fengycin and surfactin secreted from FJAT-2349 all decreased along with an increasing culture temperature. The fermentation supernatants and LP extracts had the strongest inhibition activities of Ralstonia solanacearum when strain FJAT-2349 was incubated at 25°C using potato dextrose broth as the culture medium among all the assayed culture conditions. The purified fengycin was found to be the active antibacterial compound against R. solanacearum, but the purified surfactin was not. The pot experiments demonstrated that the LPs secreted from the strain FJAT-2349 could effectively control the tomato bacterial wilt with a biocontrol efficiency of 97·6%. CONCLUSIONS: The LPs secreted from strain FJAT-2349 could serve as potential biocontrol agents against tomato bacterial wilt. SIGNIFICANCE AND IMPACT OF THE STUDY: The LPs exhibited good potential applications in the biocontrol of tomato bacterial wilt.