化学
氧化铈
电化学
组合化学
DNA
纳米颗粒
检出限
基质(水族馆)
大肠杆菌
放大器
铈
氧化物
无机化学
纳米技术
色谱法
生物化学
电极
材料科学
有机化学
生物
聚合酶链反应
物理化学
基因
生态学
作者
Hyo Yong Kim,Jun Ki Ahn,Moon Il Kim,Sun-Hee Park,Hyun Gyu Park
标识
DOI:10.1016/j.elecom.2018.12.008
摘要
We herein developed a simple and ultrafast electrochemical DNA detection method by utilizing the oxidase-mimicking activity of cerium oxide nanoparticles (CeO2). In the sensor, the presence of DNA, which electrostatically interacts with CeO2 and 3,3′,5,5′-tetramethylbenzidine (TMB) substrate, limits the formation of CeO2-TMB complex that is required for the oxidase-like reaction. As a result, the oxidase-mimicking activity of CeO2 is not effectively exerted, leading to the significantly diminished electrochemical current signal. With this novel strategy, we successfully determined DNA amplicon derived from Escherichia coli (E. coli) with the total assay time less than 6 min even without the post-purification of DNA amplicon.
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