细胞毒性
生物
嵌合抗原受体
计算生物学
体外
免疫学
T细胞
生物化学
免疫系统
作者
Amanda Lisby,Robert D. Carlson,Trevor R. Baybutt,Megan Weindorfer,Adam E. Snook
标识
DOI:10.1016/bs.mcb.2021.08.002
摘要
This chapter describes the most common method for evaluating cytotoxicity of chimeric antigen receptor (CAR) T cells, the xCELLigence real-time cell analysis (RTCA) platform (Agilent Technologies, Inc., Santa Clara, CA). Though there are a variety of assays used to evaluate conventional and engineered T cell cytotoxicity, the benefit of the xCELLigence platform is the depth of real-time data collected. This chapter begins by providing information on the conceptual basis underlying the xCELLigence assay, followed by a detailed protocol for the application of this assay to evaluate your own CAR-T cells, as well as specific insight and helpful tips for assay design, usage, and data analysis. Application of the information and methods discussed within this chapter will provide a greater understanding for evaluating cytotoxicity of CAR-T cells using this in vitro model system.
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