During the present investigation, a total of 9 genera, 3 families and 2 orders reported from the Yamuna River. Tor putitora was the most abundant fish with 20.13 percentage composition; it constitutes 0.169 abundance and 20.21 percentage relative abundance. Tor putitora was followed by Barilius bendelisis with percentage composition of 18.83, its abundance was 0.158 and with relative abundance of 18.89. Of all the fishes with Tor tor was least abundant with percentage composition of 1.29, it constitutes 0.010 abundance and 1.20% relative abundance. The fish species diversity during the study period was 0.889 which indicates the good diversity of the River Yamuna during the study period.