Effect of E Coli Expressed Recombinant Human Bone MorphogeneticProtein-2(rhBMP-2) on Stem Cells for Periodontal TissueRegeneration: A Preliminary Study

Periodontal disease results in destruction of periodontal tissues(cementum, alveolar bone and periodontal\nligament) which may ultimately lead to tooth loss. To date, there is no ideal treatment approach in achieving optimal\nperiodontal tissue regeneration. Recruitment of progenitor cells to the site which could differentiate into cementoblasts,\nosteoblasts and periodontal ligament-forming cells are needed and recently, using pluripotent stem cells isolated\nfrom various tissues in conjugation with growth and differentiation factors have been presented to lead\nperiodontal tissue regeneration. This study evaluated the effect of E coli expressed recombinant human bone morphogenetic\nprotein-2(ErhBMP-2) to periodontal ligament stem cells(PDLSCs) and bone marrow stem\ncells(BMSCs) on periodontal tissue regeneration. rhBMP-2 those expressed from E coli was treated on ex-vivo\nexpanded human BMSCs and PDLSCs to evaluate its regenerative effect in vivo and in vitro. Cementum, PDL-like\ntissues and bone were regenerated in transplanting PDLSCs and BMSCs, respectively. There was enhanced tissue\nregeneration when ErhBMP-2 was treated to PDLSCs, but not with BMSCs. Similar results were observed with in\nvitro experiments. Though further studies are needed, the results suggest a possible treatment approach in combining\nErhBMP-2 and PDLSCs for periodontal tissues regeneration.