鞭毛蛋白
奇异变形杆菌
毒力
鞭毛
微生物学
生物
细胞器
细菌
细胞生物学
生物化学
遗传学
基因
金黄色葡萄球菌
作者
María José González,Victoria Iribarnegaray,Pablo Zunino,Paola Scavone
出处
期刊:Methods in molecular biology
日期:2019-01-01
卷期号:: 35-44
被引量:1
标识
DOI:10.1007/978-1-4939-9601-8_5
摘要
Flagella are effective organelles of locomotion and one of several virulence factors in Proteus mirabilis. To study their properties and role in virulence, we describe a protocol to extract and purify the native flagellin of P. mirabilis. Purified flagellin can be visualized by SDS-PAGE or immunoblot and is suitable for downstream applications such as immunization.
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