互补
海侵隔离
等位基因
遗传学
杂种优势
生物
基因座(遗传学)
人口
大块分离分析
上位性
数量性状位点
基因
混合的
园艺
表型
基因定位
染色体
人口学
社会学
作者
Wubei Zong,Yingang Song,Dongdong Xiao,Xiaotong Guo,Fuquan Li,Kangli Sun,Wenjing Tang,Wenhao Xie,Yanqiu Luo,Liang Shan,Jun Zhou,Xianrong Xie,Dilin Liu,Letian Chen,Haiyang Wang,Yao‐Guang Liu,Jingxin Guo
摘要
Rice (Oryza sativa L.) is a short-day plant whose heading date is largely determined by photoperiod sensitivity (PS). Many parental lines used in hybrid rice breeding have weak PS, but their F1 progenies have strong PS and exhibit an undesirable transgressive late-maturing phenotype. However, the genetic basis for this phenomenon is unclear. Therefore, effective methods are needed for selecting parents to create F1 hybrid varieties with the desired PS. In this study, we used bulked segregant analysis with F1 Ningyou 1179 (strong PS) and its F2 population, and through analyzing both parental haplotypes and PS data for 918 hybrid rice varieties, to identify the genetic basis of transgressive late maturation which is dependent on dominance complementation effects of Hd1, Ghd7, DTH8, and PRR37 from both parents rather than from a single parental genotype. We designed a molecular marker-assisted selection system to identify the genotypes of Hd1, Ghd7, DTH8, and PRR37 in parental lines to predict PS in F1 plants prior to crossing. Furthermore, we used CRISPR/Cas9 technique to knock out Hd1 in Ning A (sterile line) and Ning B (maintainer line) and obtained an hd1-NY material with weak PS while retaining the elite agronomic traits of NY. Our findings clarified the genetic basis of transgressive late maturation in hybrid rice and developed effective methods for parental selection and gene editing to facilitate the breeding of hybrid varieties with the desired PS for improving their adaptability.
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