Reassembly Design of Ferritin Cages

The ferritin family is distributed in nearly all organisms and protects them from iron-induced oxidative damage. Besides, its highly symmetrical structure and biochemical features make it an appealing material for biotechnological applications, such as building blocks for multidimensional assembly, templates for nano-reactors, and scaffolds for encapsulation and delivery of nutrients and drugs. Moreover, it is of great significance to construct ferritin variants with different properties, size, and shape to further broaden its application. In this chapter, we present a routine process of the ferritin redesign and the characterization method of the protein structure to provide a feasible scheme.