脱落酸
拟南芥
磷酸化
激酶
生物
细胞生物学
下调和上调
细胞质
信号转导
拟南芥
基因
植物
遗传学
突变体
作者
Yingying Liu,Qian Zhang,D. H. Chen,Wensen Shi,Xuemeng Gao,Yu Liu,Bo Hu,Anhu Wang,Xiaohua Li,Xinyuan An,Yi Yang,Xufeng Li,Zhibin Liu,Jianmei Wang
标识
DOI:10.1016/j.jplph.2023.154165
摘要
The phytohormone abscisic acid (ABA) regulates plant growth and development and stress resistance through the ABA receptor PYLs. To date, no interaction between CPK and PYL has been reported, even in Arabidopsis and rice. In this study, we found that MdCPK4 from Malus domestica (Md for short) interacts with two MdPYLs, MdPYL2/12, in the nucleus and the cytoplasm in vivo and phosphorylates the latter in vitro as well. Compared with the wild type (WT), the MdCPK4- or MdPYL2/12-overexpressing Arabidopsis lines showed more sensitivity to ABA, and therefore stronger drought resistance. The ABA-related genes (ABF1, ABF2, ABF4, RD29A and SnRK2.2) were significantly upregulated in the overexpressing (OE) lines after ABA treatment. These results indicate that MdCPK4 and MdPYL2/12 act as positive regulators in response to ABA-mediated drought resistance in apple. Our results reveal the relationship between MdCPK4 and MdPYL2/12 in ABA signaling, which will further enrich the molecular mechanism of drought resistance in plants.
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