骨桥蛋白
骨保护素
化学
骨钙素
骨吸收
运行x2
静电纺丝
吸收
兰克尔
骨质疏松症
骨重建
纳米纤维
生物医学工程
碱性磷酸酶
材料科学
激活剂(遗传学)
成骨细胞
体外
内科学
受体
纳米技术
医学
生物化学
有机化学
酶
聚合物
作者
Yang Yong,Maolei Sun,Jia Wang,Kun Jiang,Shaoru Wang,Yun Liu,Liping Liu,Zhihui Dai,Jiang Xia,Tao Yang,Yungang Luo,Zhiqiang Cheng,Hailiang Wang,Guomin Liu
标识
DOI:10.1016/j.colsurfb.2023.113659
摘要
Osteoporotic bone defect has become clinic challenge due to its morbid bone microenvironment. Overactive bone resorption and limited bone formation lead to unstable combination between bone tissue and scaffolds. Electrospinning has been widely used in guide tissue membrane, but its barrier property results in limited application. In order to optimize the structure and add anti-bone resorption function of electrospinning fibers, we exploited the application of short fibers generated by homogenization at osteoporotic tibial bone defect. The modified nano-hydroxyapatite (m-HA) was loaded with alendronate. It overcame the problem that hydrophilic drugs were difficult to distribute uniformly in hydrophobic fibers. We confirmed that m-HA was loaded into polycaprolactone (PCL) short fibers. PCL short fibers with m-HA (PCL/m-HA) continuously released ALN, provided stable structure and showed good cytocompatibility. In vitro, PCL/m-HA increased the activity of alkaline phosphatase (ALP), promoted extracellular matrix mineralization and upregulated the expression of osteogenesis-related genes, Col 1, Alp, osteopontin (Opn) and runt-related transcription factor 2 (Runx2). In vivo, PCL/m-HA short fibers accelerated the new bone formation, inhibited the bone resorption and rebalanced the bone microenvironment through regulating osteoprotegerin (OPG) /receptor activator of NF-kB (RANKL) ratio. The above results confirmed that the PCL/m-HA short fibers achieved the application of three-dimension osteoporotic bone defect and had potential prospects in bone tissue scaffolds.
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