Naphthalimide-derived fluorogenic SNAP probe for real-time monitoring of protein degradation

We developed the fluorogenic SNAP probe BGAN-8C to monitor protein degradation. It exhibited a 6-fold fluorescence enhancement upon binding with SNAP-tag. After the SNAP protein is degraded, the fluorescence of the released probe is quenched due to aggregation. BGAN-8C was successfully employed to monitor the degradation of mODC in live cells, offering a new tool for studying protein dynamics.