Triterpenes From Lagerstroemia indica L. Bark With Anti‐ZIKV Activity

ABSTRACT Two previously undescribed triterpenes, 2 S ,3 R ,23‐trihydroxy‐1‐ oxo ‐olean‐12‐en‐28‐oic acid ( 1 ) and 2 S ,3 R ‐dihydroxy‐1‐ oxo ‐olean‐12‐en‐28‐oic acid ( 2 ), along with 19 known triterpenes ( 3–21 ), were isolated from the bark of Lagerstroemia indica L. Their structures were determined by comprehensive spectroscopic analysis, including NMR, HR‐ESI‐MS, UV, and IR measurements. The absolute configurations were established through ECD calculations. Compound 4 demonstrated potent anti‐ZIKV activity with an EC 50 value of 6.05 ± 0.51 µM. Notably, Compound 4 was shown to inhibit ZIKV envelope (E) protein expression and viral replication in a dose‐dependent manner.