Antiviral activity of type I and type III interferons against porcine reproductive and respiratory syndrome virus (PRRSV)

The newly identified type III interferons (IFNs), also known as IFN-λ1/IL-29, IFN-λ2/IL-28A and IFN-λ3/IL-28B, like type I IFNs, have antiviral activity against a broad spectrum of viruses. We therefore examined whether type III IFNs, as well as type I IFNs, has the ability to inhibit porcine reproductive and respiratory syndrome virus (PRRSV) replication in MARC-145 cells. We found that replication of PRRSV in MARC-145 cells was significantly reduced following treatment with IFN-λ1, IFN-λ2 and IFN-λ3, respectively, and such inhibition was dose-dependent. However, type III IFNs (IFN-λ1, IFN-λ2 and IFN-λ3) was less effective than type I IFNs (IFN-α and IFN-β) in antiviral activity against PRRSV. Mixture of two types of IFNs could not improve the antiviral activity of each type alone. In addition, all types of IFNs in our study were able to induce the expression of ISG56, 2',5'-OAS and MxA in MARC-145 cells. These data demonstrate that type III IFNs had antiviral activity against PRRSV and may serve as useful antiviral agents against infectious swine diseases.