破骨细胞
六烯酸
降钙素受体
CD14型
单核细胞
花生四烯酸
秩配基
内科学
生物
细胞分化
多不饱和脂肪酸
内分泌学
细胞生物学
化学
受体
生物化学
兰克尔
激活剂(遗传学)
免疫学
脂肪酸
医学
降钙素基因相关肽
酶
神经肽
基因
作者
Abe Kasonga,V. Deepak,Marlena C. Kruger
出处
期刊:PLOS ONE
[Public Library of Science]
日期:2015-04-13
卷期号:10 (4): e0125145-e0125145
被引量:44
标识
DOI:10.1371/journal.pone.0125145
摘要
An unbalanced diet can have adverse effects on health. Long chain polyunsaturated fatty acids (LCPUFAs) have been the focus of research owing to their necessity of inclusion in a healthy diet. However, the effects of LCPUFAs on human osteoclast formation and function have not been explored before. A human CD14+ monocyte differentiation model was used to elucidate the effects of an ω-3 LCPUFA, docosahexaenoic acid (DHA), and an ω-6 LCPUFA, arachidonic acid (AA), on osteoclast formation and activity. CD14+ monocytes were isolated from peripheral blood of healthy donors and stimulated with macrophage colony stimulating factor and receptor activator of nuclear factor kappa-B ligand to generate osteoclasts. Data from this study revealed that both the LCPUFAs decreased osteoclast formation potential of CD14+ monocytes in a dose-dependent manner when treated at an early stage of differentiation. Moreover, when exposed at a late stage of osteoclast differentiation AA and DHA impaired the bone resorptive potential of mature osteoclasts without affecting osteoclast numbers. AA and DHA abrogated vitronectin receptor expression in differentiating as well as mature osteoclasts. In contrast, the degree of inhibition for calcitonin receptor expression varied between the LCPUFAs with only AA causing inhibition during osteoclast differentiation. Furthermore, AA and DHA down regulated the expression of key osteoclast-specific genes in differentiating as well as mature osteoclasts. This study demonstrates for the first time that LCPUFAs can modulate osteoclast formation and function in a human primary osteoclast cell line.
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