静电纺丝
组织工程
材料科学
纳米纤维
生物医学工程
聚苯胺
导电体
傅里叶变换红外光谱
化学工程
纳米技术
复合材料
聚合物
聚合
医学
工程类
作者
Laleh Ghasemi‐Mobarakeh,Molamma P. Prabhakaran,Mohammad Morshed,Mohammad Hossein Nasr-Esfahani,Seeram Ramakrishna
出处
期刊:Tissue Engineering Part A
[Mary Ann Liebert]
日期:2009-11-01
卷期号:15 (11): 3605-3619
被引量:288
标识
DOI:10.1089/ten.tea.2008.0689
摘要
Fabrication of scaffolds with suitable chemical, mechanical, and electrical properties is critical for the success of nerve tissue engineering. Electrical stimulation was directly applied to electrospun conductive nanofibrous scaffolds to enhance the nerve regeneration process. In the present study, electrospun conductive nanofibers were prepared by mixing 10 and 15 wt% doped polyaniline (PANI) with poly (epsilon-caprolactone)/gelatin (PG) (70:30) solution (PANI/PG) by electrospinning. The fiber diameter, pore size, hydrophilicity, tensile properties, conductivity, Fourier transform infrared (FTIR), and X-ray photoelectron spectroscopy spectra of nanofibers were determined, and the in vitro biodegradability of the different nanofibrous scaffolds was also evaluated. Nanofibrous scaffolds containing 15% PANI was found to exhibit the most balanced properties to meet all the required specifications for electrical stimulation for its enhanced conductivity and is used for in vitro culture and electrical stimulation of nerve stem cells. 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and scanning electron microscopy results showed that conductive nanofibrous scaffolds are suitable substrates for the attachment and proliferation of nerve stem cells. Electrical stimulation through conductive nanofibrous PANI/PG scaffolds showed enhanced cell proliferation and neurite outgrowth compared to the PANI/PG scaffolds that were not subjected to electrical stimulation.
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