Glutathione-decorated fluorescent carbon quantum dots for sensitive and selective detection of levodopa

Levodopa has been a standard drug for treating Parkinson’s disease since the 1960s, but it has caused many side effects such as wearing-off, motor fluctuation, and dystonia. In this work, we developed glutathione-conjugated carbon quantum dots (GSH-CQDs) as a novel fluorescent sensor for sensitive and selective detection of levodopa. The GSH-CQDs were prepared by EDC/NHS coupling reaction of glutathione (GSH) with amine-functionalized CQDs (N-CQDs) synthesized using meta-phenylenediamine and ethylenediamine. The synthesized GSH-CQDs emitted bright green fluorescence with a high quantum yield (QY) of 22.42 ± 6.88%. However, upon the addition of levodopa to GSH-CQDs under alkaline conditions, the fluorescence of GSH-CQDs was quenched. Since levodopa is converted to dopaquinone in an alkaline environment, it is presumed that thiol groups of GHS-CQDs form covalent bonds with dopaquinone, causing fluorescence quenching through photoinduced electron transfer. Therefore, as the concentration of levodopa increased, the fluorescence intensity of GSH-CQDs was gradually decreased. Under optimal conditions, a linear response was observed in the range of 0.05–1 μM, and limit of detection (LOD) was determined to be 0.057 μM. The GSH-CQDs exhibited high specificity to levodopa over other non-target biological substances, quinone derivatives, and Parkinson’s medications. Furthermore, the capability of this GSH-CQDs sensor for monitoring levodopa in human serum were validated with excellent precision and recovery rates of 100.20–103.33%. • The glutathione-conjugated carbon quantum dots (GSH-CQDs) are developed for sensitive and selective detection of levodopa. • The GSH-CQDs with a high quantum yield (22.42 ± 6.88%) emit bright green fluorescence. • Levodopa is detected by the quenched fluorescence of GSH-CQDs under alkaline conditions. • The GSH-CQDs exhibit high specificity as well as superior sensitivity to levodopa. • The GSH-CQDs provide a useful strategy to detect levodopa in real samples with outstanding precision and reproducibility.