Objective To study the chemical constituents of Eurycoma longifolia Jack.Methods The compounds were isolated by chromatography on silica gel column,Sephadex LH-20 column and recrystallization methods,and their structures were determined on the basis of special analysis.Results Five compounds were obtained and identified as scopoletin(1),9-methoxycanthin-6-one(2),7-methoxy-β-carbolin-1-propionic acid(3),laurycolactone A(4),7-methoxyinfractin(5).Conclusions Compounds 1-4 are four known compounds and compound 5 is a new compound.