Modification of platelet HLA antigens and animal experiment

Objective To study the biological effect and mechanism of platelet HLA modification by mPEG-BTC and development of a universal platelet. Methods Phenotype HLA-A2 Lymphocytes as indicator cells to study platelet camouflage by mPEG-BTC, and the results were shown by microlymphocytotoxicity test. Platelet adhesion,aggregation and release were tested to show the effect of mPEG-BTC modification on platelets. The modified platelets were transfused to rabbits and monkeys. Results HLA on platelets was modified by mPEG-BTC. Platelet adhesion and aggregation increased after the modification. The platelet release function was not influenced by modification . CCI at 24 hours and platelet recovery rate showed effective transfusion and no adverse reaction in animal experiment. Conclusion HLA on platelet can be modified by mPEG-BTC, developing universal platelets is possible in the future.