Determination of lobetyolin in Radix Codonopsis from different origins by HPLC

Objective To determine the content of lobetyolin in Radix Codonopsis from different regions by HPLC.Methods HPLC was carried out on Diamonsil C18 column(250 mm×4.6 mm,5 μm) with the mobile phase acetonitrile-water(25∶75) and the UV detector was set at 268 nm.The injection volume was 20 μL.Results The calibration curve was linear in the range of 0.008 5-0.425 mg/mL(r=0.999 5),and the average recovery rate was 101.4%.The contents of lobetyolin in Radix Codonopsis from 9 origins were varied between 0.187 mg/g and 1.174 mg/g.Conclusion The method is simple,rapid and reproducible,which can be used for the quality control of Radix Codonopsis.