Optical coherence tomography(OCT) is a novel tomography imaging technique,it is an another new tomography after X-ray,CT and magnetic resonance imaging technology.OCT can detect the surface of in vivo biopsy depth of 2-3mm organizations to implement the micro-structure of three-dimensional imaging.It has a significant application in many areas of biomedicine and a wide range.The application in ophthalmology,dermatology,cardiovascular system,digestive system and in TCM research so on is described.